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Growth hormone stimulation test procedure

Growth hormone stimulation test procedure

Test supplies: blood glucose meter, blood glucose test strip, sphygmomanometer, saline 500ml, insulin injection, levodopa tablets, glucose oral solution, several syringes, several blood collection tubes

First, the extraction of venous blood
Growth hormone determination 4ml (if you need to measure liver, kidney function, nail function, etc., add the corresponding blood volume, in charge of storage), marked as 0’
In addition to 4 ml of blood for genotropin growth hormone determination, the rest of the blood is sent to the corresponding laboratory.

Second, pass saline 500ml, establish an intravenous channel

Third, blood glucose measurement (if the blood glucose value <2.6mmol / L, you can not apply insulin stimulation)

Fourth, oral levodopa, insulin injection through the established intravenous channel, and began recording time
Levodopa dosage: 10mg/kg, maximum dosage 500mg
Insulin dosage: 0.1u/kg, diluted with 2ml of normal saline

  1. After the venous blood is taken 4 ml every 30 minutes, and labeled as 30′, 60′, 90′, etc.

Sixth, the blood is completed, the test is over (the blood sample is sent to the biochemical room on the second floor of the inpatient department)

Precautions:

  1. Insulin hypoglycemia test is generally used for children >2 years old.
  2. Unexplained epilepsy, patients with a history of melanosis or who are receiving antiepileptic treatment cannot be tested.
  3. The initial blood glucose <2.6mmol/L, can not be stimulated with insulin;
  4. Close monitoring of blood sugar and blood pressure, when the blood glucose decline rate > 50% of the basic value, or the absolute value of blood sugar ≤ 2.6mmol / L, indicating that the degree of hypoglycemia reaction has been reached, the test is successful, otherwise the test fails, the next day is heavy do.
  5. Hypoglycemia treatment: Hypoglycemia usually occurs within 20 to 30 minutes of the test. If the subject has hypoglycemia such as sweating and burnout, blood glucose <2.6mmol / L, should drink drinking water, the test can still proceed as planned. If the subject is unable to take oral glucose, 200 mg/kg of glucose (10% glucose 2 ml/kg) is administered intravenously within 3 minutes. This vein channel cannot be used to collect blood samples. The intravenous drip rate was started at 4 to 8 mg/kg·min. The blood glucose concentration should be monitored after 4 to 5 minutes, and the input speed should be adjusted to maintain the blood glucose concentration at 5 to 8 mmol/L. The input continues until the end of the experiment. If necessary, give glucagon 1mg intramuscular injection, increase blood sugar, and continue to closely monitor blood sugar.
  6. Do not use any more saline water at the beginning of each blood collection.

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